Blerta Brati
Institute of Public Health, Tirana, Albania
Title: Prevalence of agr specificity groups among Staphylococcus aureus strains colonizing hospitalized patients in tertiary hospital centre
Submitted Date: November 13, 2019
Biography
Blerta Brati has a diploma from the University of Tirana, Faculty of Medicine in 1997 as a Medical Doctor and in the same year she became part of Public Health Institute in Tirana. Mean time Brati completed her study in Medical Microbiology during the years 2002-2005, Department of Microbiology Laboratory. She serves as Chief of Media Cultures Production and Anti-Sera & Antigen Diagnostics unit from 2012 until now. Brati joined the faculty of Stomatology and Faculty of Medical Science as a lecturer and was appointed as a faculty member in the Medical University Aldent, Tirana in 2014. Her interests are focused on the diagnosis of Bacterial Infection and the role of them in human disease.\r\n
Abstract
Methicillin resistant Staphylococcus aureus (MRSA) is a major pathogen in humans, and the regulator of virulence factors in S. aureus is accessory gene regulator (agr) quorum sensing system. This study aims to investigate the prevalence of the MRSA and frequency of virulence genes in hospitalized patients in tertiary hospital centre in Tirana, Albania. Latex agglutination kit for the rapid detection of PBP2 and the cefoxitin disk screen test were used for detection of MRSA in 452 samples. PCR assays were used to detect gene`s content (agr groups I-IV) in 150 clinical samples isolates. Samples were collected from blood stream and catheter-associated infections 31.2%; 27.9% from urine infections; 16.2% from skin and soft-tissue infections cases; 14% from wounds and 10.75% from nasal area. The prevalence of methicillin-resistant S. aureus (MRSA) resulted 33.2% and 12% of them demostreted multi-drug resistant with resistance to six classes of antibiotics. The most predominated agr specificity group was agr I 43%, agr II was 28%, agr III 19%, dhe agr IV 7%. It wasn`t find association between agr group with gender, age and samples origin, p value resulted >0.05. There is lack of data associate to agr group in MRSA in Albania population and this study aims to clarify the prevalence of agr groups. The majority of isolates were classified as agr group I. The obtained results do not allow us to establish a direct relationship between virulence of agr groups and some of S. aureus infection factors. So more expanded surveillance studies are needed to apply in the future for better estimation of agr virulence.\r\n
Blerta Brati
Institute of Public Health, Albania
Title: Staphylococcus aureus; Prevalence of this infection in hospitalized patients
Submitted Date: November 13, 2019
Biography
Blerta Brati has a diploma from the University of Tirana, Faculty of Medicine in 1997 as a Medical Doctor and in the same year she became part of Public Health Institute in Tirana. Mean time Brati completed her study in Medical Microbiology during the years 2002-2005, Department of Microbiology Laboratory. She serves as Chief of Media Cultures Production and Anti-Sera & Antigen Diagnostics unit from 2012 until now. Brati joined the faculty of Stomatology and Faculty of Medical Science as a lecturer and was appointed as a faculty member in the Medical University Aldent, Tirana in 2014. Her interests are focused on the diagnosis of Bacterial Infection and the role of them in human disease.\r\n
Abstract
Staphylococcus aureus is a signifi cant pathogen in human medicine. Th e prevalence of S.aureus varies between age, gender, ethnicity, geographic location, and body niche. Th e study aim is to evaluate the prevalence of S. aureus and also to describe observational result of the infl uence bacterial; host and environmental/modifi able factors might have on the relationship with humans. Th is study was carried out between October 2016 until to December 2017 across hospitalized patients from diff erent units of Mother Th eresa Hospital Center. About 258 Clinical specimens were collected based on infection type such as wound, pus/exudates, blood, urine, sputum and indwelling medical devices. We isolated and identifi ed S. aureus using standard tests like catalase, coagulase, and growth on Mannitol salt agar. Also for further accurate microbial identifi cation we have use the VITEK® 2 system. Over all 258 speciemen tested the prevalence of S. aureus was found in 36% patients. Out of all 93 cases isolated with S. aureus, 25% were from urine infections; 24.6 % from skin and soft -tissue infections cases; 20.4 from vaginal and urethral swab; 15% from nasal and ear swab cases and 15% from blood stream, indwelling medical devices and catheter-associated infections. We did found statistically signifi cant diff erences between Infection and sex, residence area, wards and place where the samples were collected. In all cases the p value was< 0.05. Th e rate of S. aureus in hospitalized patients on this study was high. Th ese results indicated that this type of infection is a signifi cant concern for health services and patients included. Th e highest percentage of S.aureus found in surgical and non surgical wound suggest that further investigation should be implemented. A screening of all hospitalized cases can lead to reduce the incidence of this infection in the hospital environment and also to control the risk factors.\r\n